Profile
Techniques, Software & Instrumentation
Biotechnology and Biochemical Techniques
Protein/Enzyme/Antibodies expression, optimization and purifications, Complex Isolations, co-IP, Density Gradient analysis DNA & RNA (total RNA, mRNA & small RNA) isolation, cDNA preparation SDS-PAGE, Urea PAGE, Native PAGE and Agarose gel electrophoresis, Southern and Western blot, Immunoprecipitation PCR (Colony and Hot Start), RT-PCR, qPCR, dPCR, Real-Time PCR; 5? RACE (Rapid Amplification of cDNA Ends), NGS Restriction-Digestion, Transformation (Electroporation), Gene Cloning, gene knockout and integration (Gibson assembly, yeast mating), in vitro transcription and translation Plant (Rice, Tomato, and Arabidopsis) Tissue Culture, Yeast (S. cerevisiae and P. pastoris) and Bacterial culture, Sorting, Yeast two-hybrid assays (ONPG; Spot Assay), Yeast Library, Enzymatic assays, Assay Development, Proliferation assays, Ligand Binding Assays, Dual-Luciferase Reporter Assays, Enzyme kinetics BiFC, FRET, FRAP, EMSA, ELISA, HPLC (Elementary, theoretical), Microscopy (Fluorescence, Confocal, TIRF), smFRET
Computer and Language Skills
Skills in Intellectual Property Law
Lean Six Sigma
Profile
Techniques, Software & Instrumentation
Biotechnology and Biochemical Techniques
Protein/Enzyme/Antibodies expression, optimization and purifications, Complex Isolations, co-IP, Density Gradient analysis DNA & RNA (total RNA, mRNA & small RNA) isolation, cDNA preparation SDS-PAGE, Urea PAGE, Native PAGE and Agarose gel electrophoresis, Southern and Western blot, Immunoprecipitation PCR (Colony and Hot Start), RT-PCR, qPCR, dPCR, Real-Time PCR; 5? RACE (Rapid Amplification of cDNA Ends), NGS Restriction-Digestion, Transformation (Electroporation), Gene Cloning, gene knockout and integration (Gibson assembly, yeast mating), in vitro transcription and translation Plant (Rice, Tomato, and Arabidopsis) Tissue Culture, Yeast (S. cerevisiae and P. pastoris) and Bacterial culture, Sorting, Yeast two-hybrid assays (ONPG; Spot Assay), Yeast Library, Enzymatic assays, Assay Development, Proliferation assays, Ligand Binding Assays, Dual-Luciferase Reporter Assays, Enzyme kinetics BiFC, FRET, FRAP, EMSA, ELISA, HPLC (Elementary, theoretical), Microscopy (Fluorescence, Confocal, TIRF), smFRET
Computer and Language Skills
Skills in Intellectual Property Law
Lean Six Sigma